Microbial profile of symptomatic pericoronitis lesions: a cross-sectional study

Abstract Objective: The microbial composition of pericoronitis (Pc) is still controversial; it is not yet clear if the microbial profile of these lesions is similar to the profile observed in periodontitis (Pd). Therefore, the aim of the present study was to describe the microbial profile of Pc lesions and compare it directly with that of subjects with Pd. Methodology: Subjects with Pc and Pd were selected, and subgingival biofilm samples were collected from (i) third molars with symptomatic Pc (Pc-T), (ii) contralateral third molars without Pc (Pc-C) and (iii) teeth with a probing depth >3 mm from subjects with Pd. Counts and proportions of 40 bacterial species were evaluated using a checkerboard DNA-DNA hybridization technique. Results: Twenty-six patients with Pc and 18 with Pd were included in the study. In general, higher levels of microorganisms were observed in Pd. Only Actinomyces oris and Eubacterium nodatum were present in higher mean counts in the Pc-T group in comparison with the Pc-C and Pd-C groups (p<0.05). The microbiota associated with Pc-T was similar to that found in Pc-C. Sites with Pc lesions had lower proportions of red complex in comparison with the Pd sites. Conclusion: The microbiota of Pc is very diverse, but these lesions harbour lower levels of periodontal pathogens than Pd.

Pericoronal lavage with chlorhexidine gluconate and povidone-iodine as a treatment for pericoronitis: a clinical and microbiological study

This study examines the effect of chlorhexidine gluconate, and povidone- iodine solutions as pericoronal lavage and mouth rinse on patients with score 2 or 3 pericoronitis. Patients were divided at random into three groups. Group 1 irrigated with chlorhexidine gluconate, group 2 irrigated with povidone-iodine, and group 3 irrigated with normal saline and served as control. Lavage was performed once daily for two consecutive days. Every patient was rinsing with the same irrigant t.d.s for 5 days. Bacteriologic samples for culture and Gram's staining were taken from the pericoronal pouches of lower third molar teeth before and after lavage at 2 consecutive days. Pericoronal index scores were recorded for every patient before and after treatment. Results have shown that the predominant microflora of the 80 examined patients were highly anaerobic, and that both chlorhexidine gluconate and povidone iodine significantly reduced the degree of pericoronal inflammation and bacterial counts. No difference was found between the two agents. It can be concluded that local antimicrobial irrigating solutions when used as pericoronal lavage can effectively treat cases of score 2 and score 3 pericoronitis without the need for systemic antibiotics

Bactérias anaeróbias associadas à pericoronarite / Anaerobic bacteria associated to \"pericoronarite\"

A microbiota anaeróbia associada a casos de pericoronarite foi estudada. Espécimes de 15 pacientes com pericoronarite e de 15 indivíduos sadios foram coletados. O isolamento microbiano foi realizado em ágar sangue com hemina e menadiona e meios seletivos Omata & Disraely e TSBV, incubados em condições de anaerobiose, a 37ºC, por 10, 4 e 3 dias respectivamente. Os isolados eram escolhidos ao acaso, e identificados de acordo com suas características morfocoloniais, citológicas e por meio de testes bioquímico-fisiológicos. Os anaeróbios obrigatórios foram os mais frequentemente recuperados, em especial as fusobactérias bucais e bastonetes anaeróbios produtores de pigmento negro (Prevotella e Porphyromonas). Os resultados mostram que a microbiota associada à pericoronarite é semelhante àquela envolvida na doença periodontal